|
PatentVote.com: Vote on your favourite invention!
|
|
 |
Title: Reagent and method for determining total calcium in body fluids
Claims: I claim: 1. A method for the rapid determination of total calcium in protein-containing body fluids, comprising the steps of mixing a sample of body fluid with a color reagent consisting essentially of an aqueous solution of chlorophosphonazo III buffered to a pH within the range of 5.0 to 5.5 by a buffer comprising potassium hydrogen phthalate, and thereafter optically comparing the color development of the sample-reagent mixture with that of the same reagent mixed with a standard of known calcium concentration. 2. The method of claim 1 wherein the pH of said color reagent falls within the range of 5.35 to 5.45. 3. The method of claim 1 in which the pH of said color reagent is about 5.4. 4. A reagent for rapidly determining total calcium in a protein-containing body fluid, comprising an aqueous solution of chlorophosphonazo III buffered to a pH within the range of 5.0 to 5.5, said reagent including potassium acid phthalate as part of the buffer system thereof. 5. The reagent of claim 4 in which the buffer system also includes potassium hydroxide. 6. The reagent of claim 4 in which said pH is within a range of 5.35 to 5.45. 7. The reagent of claim 4 in which said pH is about 5.4. Other info: Inventors: Cleaver, Kenneth E. (Woodbury, NJ, US) Application Number: 496003 Filing Date: 1974-08-09 Publication_date: 1976-01-27 Assignee: American Hospital Supply Corporation (Evanston, IL) Primary Class(es): 436/74 436/79 Other Classes: US Patent Ref: Other Refs: Other References: ferguson et al., Anal. Chem. 36, 796, (1964). Hackh's Chemical Dictionary, 4th Edn., McGraw-Hill, p. 538 relied on, 1969. | |