Title: Reagent for the determination of β-lactamase

Abstract: The present invention provides a process for the determination of β-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen.

The present invention also provides a reagent for the determination of β-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.

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Inventors: Stahl, Peter (Bernried, DE)
Vomel, Wolfgang (Mannheim, DE)
Seidel, Hans (Tutzing, DE)

Application Number: 06/265267
Filing Date: 1981-05-20
Publication_date: 1983-05-10
Assignee: BOEHRINGER MANNHEIM GMBH (DE)

Primary Class(es): 435/23 435/805, 435/34, 435/810, 435/27, 435/25, 435/39
Other Classes: C12Q1/34; C12Q1/30; C12Q1/38; C12Q1/06; C12Q1/04
US Patent Ref:

4225669	September, 1980	Melnick et al.	435/29	Staining and analysis of bacteria
4234683	November, 1980	McMillan	435/29	Beta-lactamase diagnostic product and method
4311794	January, 1982	Melnick et al.	435/27	Determination of bacterial growth activity and antibiotic sensitivity by catalase measurement

Other Refs: Other References: Madler et al., Applied and Environmental Microbiology, 32(4), 575-578 (1976).

Primary Examiner: Kepplinger, Esther M.
Assistant Examiner:
Attorney: Felfe & Lynch

Claims:

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