Title: Process for the selective formation of disulfide bridges in polypeptides and therapeutic compositions

Abstract: A process for the selective formation of at least two disulfide bridges in a polypeptide which process comprises treating a polypeptide starting material carrying at least two SH-groups to mask at least one of said SH-groups with a p-methoxybenzyl protective group and to mask at least one of the SH-groups with an acetamido methyl protective group, then splitting-off said p-methoxybenzyl protective group with pyridine-polyhydrogen fluoride in the presence of anisol, and then treating with iodine in an acid solution to produce said disulfide bridge-containing polypeptide.

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Inventors: Birr, Christian (Leimen/St. Ilgen, DE)

Application Number: 06/158021
Filing Date: 1980-06-09
Publication_date: 1982-09-28
Assignee: Max, Planck Gesellschaft

Primary Class(es): 530/336 930/DIG.621, 530/303, 930/DIG.620, 930/260
Other Classes: C07K1/06; C07K1/113; C07K1/12; C07K14/62; A61K38/00; C07K1/00; C07K14/435; C07C103/52
US Patent Ref:

B471617	February, 1976	Kamber et al.	260/112.7
3883500	May, 1975	Geiger et al.	260/112.7	Process for the manufacture of insulin, analogs and derivatives thereof
4029642	June, 1977	Obermeier	260/112.7	Process for the manufacture of human insulin

Other Refs:
Primary Examiner: Phillips, Delbert R.
Assistant Examiner:
Attorney: Felfe & Lynch

Claims:

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