Title: Method for producing tripdiolide, triptolide and celastrol

Abstract: A process for the production of tripdiolide, triptolide and celastrol comprises the steps of:

(a) preparing a cellular inoculum from Tripterygium wilfordii Hook F;

(b) inoculating a nutrient growth medium with the cellular inoculum and incubating the inoculated growth medium at 20°-30° C. for up to 8 weeks to produce a cellular product;

(d) isolating tripdiolide, triptolide and celastrol from the cellular product and supernatant inoculated growth medium.

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Inventors: Chalmers, William T. (Vancouver, CA)
Kutney, James P. (Vancouver, CA)
Salisbury, Phillip J. (Vancouver, CA)
Stuart, Kenneth L. (Vancouver, CA)
Townsley, Phillip M. (Vancouver, CA)
Worth, Brian R. (Vancouver, CA)

Application Number: 06/165336
Filing Date: 1980-07-02
Publication_date: 1982-05-04
Assignee: Health US.

Primary Class(es): 435/126 514/571, 435/127, 514/419
Other Classes: C12P15/00; C12P17/02; C12N5/00; C12N15/00
US Patent Ref:

4005108

January, 1977

Kupchan et al.

424/279

Other Refs: Other References: Reynolds et al., "Plant Cell Lines", Methods in Enzymology, vol. LVIII, Academic Press, New York, 478-486, (1979).
Bergmann, "Plating of Plant Cells", Plant Tissue Culture and Its Bio-Technological Application, Springer-Verlag, New York, 213, 218, (1977).
Schechter et al., J.A.C.S., 64, 182-183, (1942).
Monache et al., J.C.S. Perkin I, 3127-3131, (1979).

Primary Examiner: Kepplinger, Esther M.
Assistant Examiner:
Attorney: Roberts Jr., John S.

Claims:

We cla