Title: Apparatus and method for coordinating chromatographic separation (HPLC) with UV/VIS absorbency values and with bioautograph test results

Abstract: An apparatus is described which is adaptable for use in coordinating UV/VIS absorbency values of a chromatography eluate containing antimicrobial constituents with bioautograph test results. Such an apparatus is comprised of a UV/VIS spectrophotometer equipped with a continuous recording monitor, a pair of parallel metering valves, a stream splitter, a rotatable drum adapted to hold a piece of absorbent material and means to rotate said drum. The method involves the use of such an apparatus in combination with the bioautograph test to coordinate said absorbency valves with the test results from a plurality of bioautograph test organisms.

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Inventors: Neuss, Norbert (Indianapolis, IN, US)
Miller, Roger D. (Mooresville, IN, US)

Application Number: 865278
Filing Date: 1977-12-28
Publication_date: 1979-11-20
Assignee: Eli Lilly and Company (Indianapolis, IN)
Primary Class(es): 435/32 210/198.2, 422/70, 435/288.6, 435/288.7
Other Classes:
US Patent Ref:

3700562	Oct, 1972	Morgenstern et al.	195/127.
3902971	Sep, 1975	Fletcher et al.	195/127.
4013418	Mar, 1977	Plakas	195/127.

Other Refs:
Primary Examiner: Jones, Raymond N.
Assistant Examiner: Warden, Robert J.
Attorney: Swenson; Houston L., Whale; Arthur R.