Title: Continuous cell culture system

Abstract: A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow shaft means journaled for rotation in a stationary sleeve sub-assembly and a filter unit suspended downwardly therefrom, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means and adapted for coaxial rotation with said shaft means.

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Inventors: Tolbert, William R. (Manchester, MO, US)
Feder, Joseph (University City, MO, US)
Kimes, Richard C. (Creve Coeur, MO, US)

Application Number: 850987
Filing Date: 1977-11-14
Publication_date: 1979-09-04
Assignee: Monsanto Company (St. Louis, MO)
Primary Class(es): 435/297.3
Other Classes:
US Patent Ref:

3647632

Mar, 1972

Johnson et al.

195/142.

Other Refs:
Primary Examiner: Tanenholtz, Alvin E.
Assistant Examiner: Warden, Robert J.
Attorney: Meyer; Scott J., Williams, Jr.; James W.