Title: Photometric method for the quantitative determination of a material or substance in an analysis substance and photoelectric photometer for the performance of the aforesaid method

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Abstract: A photometric method and photoelectric photometer for the quantitative determination of a substance or material in an analysis substance, wherein the course of the calibration curve representing the functional dependency of an optical measurement magnitude upon the concentration of the substance can be at least approximately determined by at most a number of few parameters. For a calibration curve parameter there is provided a reference sample which contains the substance to be analyzed in a known or unknown concentration. A partial quantity is removed from the analysis substance to serve as the analysis sample and there are formed from further partial quantities of the analysis substance corresponding to the number of further calibration curve parameters calibration samples by the addition of predetermined graduated quantities of the substance to be analyzed to the partial quantities of the analysis substance, so that each calibration sample contains the material to be analyzed in a concentration which is composed of the unknown proportion and a known proportion. The analysis sample, the reference sample and the calibration sample(s) are illuminated and for all samples there are at least approximately simultaneously determined the values of the optical measurement magnitudes, and by means of the measurement magnitude values there is mathematically determined from the course of the calibration curve determined by such measurement magnitude values and the known concentration of the material to be analyzed in the reference sample the concentration of the material or substance in the analysis sample.


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