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Primary Examiner: Schain, Howard E.
Assistant Examiner:
Attorney: Silverstein; M. Howard, Hensley; Max D., Takacs; William

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Title: Preparation of soluble edible protein from leafy green crops

Abstract: Juice obtained from alfalfa or other leafy green crops is processed to isolate a cream-colored protein fraction which is soluble, odorless, bland (essentially tasteless) and useful for human nutrition. The juice is first treated to remove chloroplastic proteins, chlorophyll, carotenoids, and lipids. The residual juice containing the desired cytoplasmic proteins is treated by novel procedures--e.g., acid precipitation in the cold or by membrane filtration followed by acid precipitation at ambient temperature--to isolate the soluble bland protein.


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Inventors: Bickoff, Emanuel M. (Oakland, CA, US)
DE Fremery, Donald (Orinda, CA, US)
Edwards, Richard H. (Albany, CA, US)
Knuckles, Benny E. (San Pablo, CA, US)
Kohler, George O. (El Cerrito, CA, US)
Miller, Raymond E. (Richmond, CA, US)

Application Number: 486703
Filing Date: 1974-07-08
Publication_date: 1976-05-25
Assignee: The United States of America as represented by the Secretary of (Washington, DC)
Primary Class(es): 530/379
Other Classes:
US Patent Ref:
2600903Jun, 1952Miller426/311.
3637396Jan, 1971Hollo et al.260/112.
3684520Aug, 1972Bickoff et al.260/112.
3823128Jul, 1974Bickoff et al.260/112.

Other Refs: Other References: J. of Biol. Chem., Vol. 49, 1921, pp. 63-91, Osborne et al.
J. Sci. Food Agric., July, 1961, pp. 502-512, Chayen et al.