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Primary Examiner: Monacell, A. Louis
Assistant Examiner: Wiseman, Thomas G.
Attorney: Montague; Ernest G., Ross; Karl F., Dubno; Herbert

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Title: Method of immobilizing enzymes to microbial cells

Abstract: Microorganic cells or microorganic cells which have undergone various forms of treatments, when treated with a reagent having two or mroe functional groups, give rise to microorganic cells having one of the functional groups of the said reagent chemically bonded thereto and also retaining a free functional group. Contact of an enzyme with the microorganic cells having a free functional group results in immobilization of the enzyme on the said cells.


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Inventors: Takasaki, Yoshiyuki (Chiba, JA)

Application Number: 447086
Filing Date: 1974-03-01
Publication_date: 1976-04-13
Assignee: Agency of Industrial Science & Technology (Tokyo, JA)
Primary Class(es): 435/174
Other Classes:
US Patent Ref:
3553310Jan, 1971Csizmas et al.195/63.
3779869Dec, 1973Zienty195/68.
3843442Oct, 1974Moskowitz195/31.

Other Refs: Other References: Hough et al, Nature Vol. 235, (Feb., 1972), p. 389.
Zaborsky, Immobilized Enzymes, CRC Press, (1973), pp. 10, 11, 64.
Barker et al., "Enzyme Reactors for Industry," Process Biochemistry, Vol. 6, No. 10, pp. 11-13.
Emery et al, "Some Applications of Solid-Phase Enzymes in Biological Engineering," Chem. Engineer, Birmingham University, Vol. 22, No. 2, pp. 37-45.