Title: Purification of d-aminoacid oxidase

Abstract: Process for the separation and purification of d-aminoacid oxidase by affinity chromatography. The process comprises contacting a mixture of proteins containing the d-aminoacid oxidase with a mineral carrier carrying haloalkylsilane grafts, upon which are fixed -- NH -- (CH.sub.2).sub.3 -- NH -- (CH.sub.2).sub.2 C.sub.6 H.sub.4 COOH residues. The enzyme retained by the carrier is then separated by a solution of ethyleneglycol. Use of the pure d-aminoacid oxidase for the preparation of cetonic acids.

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Inventors: Mazarguil, Honore (Ramonville-St. Agne, FR)
Meiller, Francois (Palaiseau, FR)
Monsan, Pierre (Toulouse, FR)

Application Number: 568797
Filing Date: 1975-04-16
Publication_date: 1976-03-02
Assignee: Rhone-Poulenc Industries (Courbevoie, FR)
Primary Class(es): 435/189 435/814
Other Classes:
US Patent Ref:

3652761	Mar, 1972	Weetall	195/DIG.
3746622	Jul, 1973	Nishikawa et al.	195/66.

Other Refs:
Primary Examiner: Shapiro, Lionel M.
Assistant Examiner:
Attorney: McDougall, Hersh & Scott