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Primary Examiner: Reese, Robert M.
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Attorney: Dawson, Tilton, Fallon & Lungmus

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Title: Reagent and method for determining total calcium in body fluids

Abstract: A colorimetric determination of total calcium in serum and other body fluids comprising the steps of mixing the sample of body fluid with a liquid reagent consisting of 2,7-bis-(4 chloro-2-phosphonobenzeneazo)-1,8-dihydroxynapthalene-3,6-disulfonic acid, commonly known as chlorophosphonazo III, in a buffer system at pH 5.0 to 5.5, and thereafter spectrophotometrically measuring the level of color development. Potassium hydrogen phthalate is disclosed as a component of the buffer system.


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Inventors: Cleaver, Kenneth E. (Woodbury, NJ, US)

Application Number: 496003
Filing Date: 1974-08-09
Publication_date: 1976-01-27
Assignee: American Hospital Supply Corporation (Evanston, IL)
Primary Class(es): 436/74 436/79
Other Classes:
US Patent Ref:
Other Refs: Other References: ferguson et al., Anal. Chem. 36, 796, (1964).
Hackh's Chemical Dictionary, 4th Edn., McGraw-Hill, p. 538 relied on, 1969.