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Title:
Reagent and method for determining total calcium in body fluids
Abstract:
A colorimetric determination of total calcium in serum and other body fluids comprising the steps of mixing the sample of body fluid with a liquid reagent consisting of 2,7-bis-(4 chloro-2-phosphonobenzeneazo)-1,8-dihydroxynapthalene-3,6-disulfonic acid, commonly known as chlorophosphonazo III, in a buffer system at pH 5.0 to 5.5, and thereafter spectrophotometrically measuring the level of color development. Potassium hydrogen phthalate is disclosed as a component of the buffer system.
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Inventors:
Cleaver, Kenneth E. (Woodbury, NJ, US)
Application Number:
496003
Filing Date: 1974-08-09 Publication_date: 1976-01-27 Assignee:
American Hospital Supply Corporation (Evanston, IL)
Primary Class(es):
436/74
436/79
Other Classes:
US Patent Ref:
Other Refs:
Other References:
ferguson et al., Anal. Chem. 36, 796, (1964). Hackh's Chemical Dictionary, 4th Edn., McGraw-Hill, p. 538 relied on, 1969. |