Title: Process for preparing intracellular substances of microbial origin

Abstract: Intracellular substances of microbial origin are prepared by culturing a microorganism in a suitable nutrient medium therefor in the presence of at least one antibiotic in sufficient amount to weaken the walls of the microorganism cells but insufficient to arrest growth of the microorganism and induce discharge of the intracellular substances into the culture medium, thereafter rupturing the walls of at least a part of the microorganism cells and recovering the intracellular substances released by rupture of the cell walls.

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Inventors: Dasek, Jaroslav (Yverdon, CH)
Traelnes, Knut Rude (Renens, CH)

Application Number: 355014
Filing Date: 1973-04-27
Publication_date: 1976-01-20
Assignee: Societe d'Assistance Technique pour Produits Nestle S.A. (Lausanne, CH)
Primary Class(es): 435/259 435/244, 435/814, 435/822, 435/837, 435/853
Other Classes:
US Patent Ref:

3243354	Mar, 1966	Nakao et al.	195/28.
3275610	Sep, 1966	Coty	195/104.
3359177	Dec, 1967	Nara et al.	195/28.
3616211	Oct, 1971	Pietsch	195/28.
3719754	Mar, 1973	Day et al.	195/28.

Other Refs:
Primary Examiner: Tanenholtz, Alvin E.
Assistant Examiner:
Attorney: Watson Leavenworth Kelton & Taggart