Title: Method of manufacturing a bacterial preparation consisting of a normal rumen bacterial flora with an improved ability to utilize ammonium salts

Abstract: A method of manufacturing a bacterial preparation for ruminants which comprises taking out ruminant-stomach bacterial groups from the rumen of a ruminant grown under an excellent condition, repeatedly inoculating by the streak method and culturing the bacterial groups on a same selective medium containing an ammonium salt, gradually increasing the medium while serially passing the bacteria on the medium, allowing certain microorganisms of the bacterial groups to utilize the metabolic products of the bacteria, thereby obtaining bacteria having an improved ammonium-utilizing ability, and then using the improved bacteria as a principal ingredient of a bacterial preparation.

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Inventors: Sakurai, Nobuo (Chiba, JA)

Application Number: 363558
Filing Date: 1973-05-24
Publication_date: 1976-01-13
Assignee: Miyairi Kinzai Kenkyusho Company Limited (JA)
Primary Class(es): 426/2 435/245, 435/440, 435/842, 435/853, 435/855, 435/885
Other Classes:
US Patent Ref:

2700611	Jan, 1965	Jeffreys	426/71.
3243299	Mar, 1966	Mecho	426/61.
3857971	Dec, 1974	Abdo et al.	426/53.

Other Refs:
Primary Examiner: Shaprio, Lionel M.
Assistant Examiner:
Attorney: Haight; James C.