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Primary Examiner: Shapiro, Lionel M.
Assistant Examiner:
Attorney: Wenderoth, Lind & Ponack

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Title: Neutral protease useful for animal tissue and cell culture

Abstract: This invention relates to a neutral protease useful for animal tissue cell culture and having the chemical and physical properties disclosed in the body of the specification, characterized in that said protease is produced by aerobically culturing a strain of Bacillus polymyxa deposited at the American Type Culture Collection, Rockville, Maryland under accession number ATCC 21993 on a culture medium containing a suitable carbon source and nitrogen source.

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Inventors: Irie, Yoshio (Matsudo, JA)

Application Number: 474771
Filing Date: 1974-05-30
Publication_date: 1976-01-06
Assignee: Godo Shusei Kabushiki Kaisha (Tokyo, JA)
Primary Class(es): 435/221 435/838
Other Classes:
US Patent Ref:
Other Refs: Other References: keay et al., Biotechnology and Bioengineering Vol. 12, pp. 179-212, (1970).
Griffin et al., Biochemical Journal, Dec. 1971, Vol. 125, No. 4, p. 109P.